The use of gel electrophoresis of seed protein in phylogeny is supported by the fact that mature seeds possess the same protein components unchanged with age or environmental stress, and thus provide valid evidence for genetic relatedness (Crawford 1990). Electrophoretic analysis of seed storage proteins was used in testing genetic associations in Vicia at generic, specific and intraspecific levels, along with morphological characterization (Ladizinsky and Hymowitz 1979 Mirali et al. Considerable insight has been drained as to their structure and synthesis during seed development and to their role as storage proteins (Higgins 1984). Seed proteins are physiologically stable and easy to manipulate (Ladizinsky and Hymowitz 1979). Morphological approach is rather difficult to estimate the all genetic diversity in the genus (Haider and El-Shanshoury 2000). Section Cracca sensu Kupicha ( 1976) belongs to subgenus Vicilla. The subgenus Vicia sensu Maxted ( 1993) contains 9 sections including sections Vicia, Hypechusa and Narbonensis. Kupicha ( 1976) has subdivided the genus into two subgenera ( Vicilla, Vicia) which have been further subdivided into 17 and 5 sections, respectively. Maxted ( 1993) pointed out that there had been 20 major classifications of the group since Linnaeus. Vetch seeds contain more than 20% crude protein and relatively high amount of lysine, leucine, arginine, phenylalanine and tyrosine (Darre et al. The genus Vicia has the capacity to fix atmospheric nitrogen (Nemecek et al.
Xlstat 2014.5.03 older version series#
In Algeria, there are 26 species belonging to three series (Quézel and Santa 1962). comprises about 210 species widely distributed along Europe, Asia and the American regions (Hanelt and Mettin 1989). The genus Vicia belongs to the Legumes, family Leguminosae which is considered one of the largest families of flowering plants and represents tremendous morphological, ecological and genetic diversity. Data obtained from ecogeographic investigation can be used for future collecting missions. No clear relationships were seen between the groups according to their geographical origin. Several accessions present specific bands which could be used as a discriminatory marker both on intra and interspecific levels. The results reflect the great diversity of storage proteins and a high correlation was obtained between the three studied fractions. Five clusters were generated from albumins, six from globulins and four from prolamins patterns. Dendrograms based on the Jaccard index and the UPGMA method were generated and the degree of genetic diversity between and within taxa was evaluated. 131, 119 and 98 bands were respectively used for albumin, globulin and prolamin cluster analysis. taxa for seed albumins, globulins and prolamins patterns by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) along with an ecogeographic characterization of sites investigated. Genetic variability was studied in 78 populations of locally collected Vicia L.
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